HAMP ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Intra-Assay Precision: %CV <4.7%, n=20, Mean Inter-Assay Precision: %CV <5.4%, n=20_x005F
CAS Number
7732-18-5
Gene Name
Hepcidin antimicrobial peptide
Gene Aliases
Hepc; hepcidin.
Gene ID
84604
Accession Number
NP_445921.1
Reactivity
Rat, Rattus norvegicus
Target
Hepcidin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HAMP ELISA Kit (Rat) (OKEH03433) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Hamp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hamp and incubated. The Hamp found in the sample or standards competes with the biotinylated Hamp for limited binding sites on the immobilized anti-Rat Hamp antibody. Excess unbound biotinylated Hamp and sample or standard Hamp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hamp captured in the well and inversely proportional to the amount of Hamp which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
9 kDa
Specificity
Natural and recombinant Rat Hepcidin
Protein Length
84
NCBI Gene Symbol
Hamp
Protein Name
Q99MH3
Gene Name URL
Hamp
Nucleotide Accession Number
NM_053469.1
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