APEX1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Apurinic/apyrimidinic endodeoxyribonuclease 1
Gene Aliases
AP endonuclease 1; APE; APEN; Apex; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; apurinic/apyrimidinic endonuclease 1; Apurinic-apyrimidinic endonuclease 1; DNA- (apurinic or apyrimidinic site) endonuclease; DNA- (apurinic or apyrimidinic site) lyase; redox factor-1; REF-1.
Gene ID
79116
Accession Number
NP_077062.1
Reactivity
Rat, Rattus norvegicus
Target
DNA- (apurinic or apyrimidinic site) lyase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology APEX1 ELISA Kit (Rat) (OKEH03429) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apex1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Apex1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apex1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
36 kDa
Specificity
Natural and recombinant Rat DNA- (apurinic or apyrimidinic site) lyase
Protein Length
317
NCBI Gene Symbol
Apex1
Protein Name
P43138
Gene Name URL
Apex1
Nucleotide Accession Number
NM_024148.1
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