What is the catalog number for BTK ELISA Kit (Mouse)?

The catalog number for BTK ELISA Kit (Mouse) is OKEH03422-96W.

What sizes are available for BTK ELISA Kit (Mouse)?

BTK ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is BTK ELISA Kit (Mouse) in stock?

BTK ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

BTK ELISA Kit (Mouse)

Selected Variant

OKEH03422-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Bruton agammaglobulinemia tyrosine kinase

Gene Aliases

Agammaglobulinaemia tyrosine kinase; agammaglobulinemia tyrosine kinase; AI528679; ATK; B-cell progenitor kinase; BPK; bruton tyrosine kinase; Bruton's tyrosine kinase; kinase EMB; tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB) ; xi; xid; X-linked immune deficiency.

Gene ID

12229

Accession Number

NP_038510.2

Reactivity

Mouse, Mus musculus

Target

Tyrosine-protein kinase BTK

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology BTK ELISA Kit (Mouse) (OKEH03422) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Btk has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Btk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Btk captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.092 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

76 kDa

Specificity

Natural and recombinant Mouse Tyrosine-protein kinase BTK

Protein Length

659

NCBI Gene Symbol

Btk

Protein Name

P35991

Gene Name URL

Btk

Nucleotide Accession Number

NM_013482.2

Price

Frequently Asked Questions

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