GSTO1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Glutathione S-transferase omega 1
Gene Aliases
Glutathione S-transferase omega 1-1; Glutathione-dependent dehydroascorbate reductase; Monomethylarsonic acid reductase; S- (Phenacyl) glutathione reductase.
Gene ID
114846
Accession Number
NP_001007603.1
Reactivity
Rat, Rattus norvegicus
Target
Glutathione S-transferase omega-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GSTO1 ELISA Kit (Rat) (OKEH03399) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gsto1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gsto1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gsto1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Rat Glutathione S-transferase omega-1
Protein Length
241
NCBI Gene Symbol
GSTO1
Protein Name
Q9Z339
Gene Name URL
GSTO1
Nucleotide Accession Number
NM_001007602.1
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