MCAM ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Melanoma cell adhesion molecule
Gene Aliases
CD146; cell surface glycoprotein MUC18; gicerin; l-gicerin; Melanoma cell adhesion molecule; melanoma-associated antigen MUC18; Muc18.
Gene ID
78967
Accession Number
NP_001029181.1
Reactivity
Rat, Rattus norvegicus
Target
Cell surface glycoprotein MUC18
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MCAM ELISA Kit (Rat) (OKEH03394) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mcam has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mcam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mcam captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum: 80%
Sensitivity
0.079 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
71 kDa
Specificity
Natural and recombinant Rat Cell surface glycoprotein MUC18
Protein Length
648
NCBI Gene Symbol
Mcam
Protein Name
Q9EPF2
Gene Name URL
Mcam
Nucleotide Accession Number
NM_001034009.1
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