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VASN ELISA Kit (Human)

Selected Variant

OKEH03392-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Vasorin

Gene Aliases

Protein slit-like 2; SLITL2; slit-like 2; vasorin.

Gene ID

114990

Accession Number

NP_612449.2

Reactivity

Homo sapiens, Human

Target

Vasorin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology VASN ELISA Kit (Human) (OKEH03392) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VASN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for VASN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VASN captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%

Sensitivity

0.177 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

72 kDa

Specificity

Natural and recombinant Human Vasorin

Protein Length

673

NCBI Gene Symbol

VASN

Protein Name

Q6EMK4

Gene Name URL

VASN

Nucleotide Accession Number

NM_138440.2

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