What is the catalog number for HMGB2 ELISA Kit (Mouse)?

The catalog number for HMGB2 ELISA Kit (Mouse) is OKEH03371-96W.

What sizes are available for HMGB2 ELISA Kit (Mouse)?

HMGB2 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is HMGB2 ELISA Kit (Mouse) in stock?

HMGB2 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

HMGB2 ELISA Kit (Mouse)

Selected Variant

OKEH03371-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

High mobility group box 2

Gene Aliases

C80539; high mobility group protein 2; high mobility group protein B2; Hmg2; HMG-2.

Gene ID

97165

Accession Number

NP_032278.1

Reactivity

Mouse, Mus musculus

Target

High mobility group protein B2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HMGB2 ELISA Kit (Mouse) (OKEH03371) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hmgb2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hmgb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hmgb2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

Sensitivity

0.085 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

24 kDa

Specificity

Natural and recombinant Mouse High mobility group protein B2

Protein Length

210

NCBI Gene Symbol

Hmgb2

Protein Name

P30681

Gene Name URL

Hmgb2

Nucleotide Accession Number

NM_008252.3

Price

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

A06034-1 100 µg/Vial