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GBA ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Glucosidase, beta, acid

Gene Aliases

Acid beta glucosidase; Acid beta-glucosidase; betaG; betaGC; beta-glucocerebrosidase; cholesterol glucosyltransferase; cholesteryl-beta-glucosidase; D-glucosyl-N-acylsphingosine glucohydrolase; GBA1; GC; GCa; GCase; GLUC; glucocerebrosidase; glucosylceramidase; lysosomal acid GCase; lysosomal acid glucosylceramidase; SGTase.

Gene ID

14466

Accession Number

NP_001070879.1

Reactivity

Mouse, Mus musculus

Target

Glucosylceramidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GBA ELISA Kit (Mouse) (OKEH03366) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gba has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gba is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gba captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%

Sensitivity

0.168 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

58 kDa

Specificity

Natural and recombinant Mouse Glucosylceramidase

Protein Length

515

NCBI Gene Symbol

Gba

Protein Name

P17439

Gene Name URL

Gba

Nucleotide Accession Number

NM_001077411.2

Frequently Asked Questions

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