SLC31A1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Solute carrier family 31 member 1
Gene Aliases
Copper transporter 1; Copper uptake transporter 1; Ctr1; high affinity copper uptake protein 1; liver regeneration-related protein LRRGT00200; LRRGT00200; rCTR1; solute carrier family 31 (copper transporter), member 1; solute carrier family 31 (copper transporters), member 1; Solute carrier family 31 member 1.
Gene ID
171135
Accession Number
NP_598284.1
Reactivity
Rat, Rattus norvegicus
Target
High affinity copper uptake protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SLC31A1 ELISA Kit (Rat) (OKEH03359) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc31a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc31a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc31a1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.172 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Rat High affinity copper uptake protein 1
Protein Length
187
NCBI Gene Symbol
Slc31a1
Protein Name
Q9JK41
Gene Name URL
Slc31a1
Nucleotide Accession Number
NM_133600.1
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