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SLC31A1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Solute carrier family 31 member 1

Gene Aliases

Copper transporter 1; Copper uptake transporter 1; Ctr1; high affinity copper uptake protein 1; liver regeneration-related protein LRRGT00200; LRRGT00200; rCTR1; solute carrier family 31 (copper transporter), member 1; solute carrier family 31 (copper transporters), member 1; Solute carrier family 31 member 1.

Gene ID

171135

Accession Number

NP_598284.1

Reactivity

Rat, Rattus norvegicus

Target

High affinity copper uptake protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SLC31A1 ELISA Kit (Rat) (OKEH03359) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc31a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc31a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc31a1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.172 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

21 kDa

Specificity

Natural and recombinant Rat High affinity copper uptake protein 1

Protein Length

187

NCBI Gene Symbol

Slc31a1

Protein Name

Q9JK41

Gene Name URL

Slc31a1

Nucleotide Accession Number

NM_133600.1

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