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CD70 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

CD70 molecule

Gene Aliases

CD27 ligand; CD27L; CD27-L; CD27LG; CD70 antigen; Ki-24 antigen; LPFS3; surface antigen CD70; TNFSF7; TNLG8A; tumor necrosis factor ligand 8A; tumor necrosis factor ligand superfamily member 7.

Gene ID

970

Accession Number

NP_001243.1

Reactivity

Homo sapiens, Human

Target

CD70 antigen

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CD70 ELISA Kit (Human) (OKEH03355) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD70 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CD70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD70 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

0.095 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

21 kDa

Specificity

Natural and recombinant Human CD70 antigen

Protein Length

193

NCBI Gene Symbol

CD70

Protein Name

P32970

Gene Name URL

CD70

Nucleotide Accession Number

NM_001252.4

Frequently Asked Questions

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