GCKR ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Glucokinase regulator
Gene Aliases
FGQTL5; GKRP; glucokinase (hexokinase 4) regulator; glucokinase regulatory protein; hexokinase 4 regulator.
Gene ID
2646
Accession Number
NP_001477.2
Reactivity
Homo sapiens, Human
Target
Glucokinase regulatory protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GCKR ELISA Kit (Human) (OKEH03348) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GCKR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for GCKR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GCKR captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
40.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
69 kDa
Specificity
Natural and recombinant Human Glucokinase regulatory protein
Protein Length
625
NCBI Gene Symbol
GCKR
Protein Name
Q14397
Gene Name URL
GCKR
Nucleotide Accession Number
NM_001486.3
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