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DRD1 ELISA Kit (Mouse)

Selected Variant

OKEH03336-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Dopamine receptor D1

Gene Aliases

C030036C15Rik; D (1A) dopamine receptor; D1 receptor; D1A dopamine receptor; dopamine D1 receptor; dopamine receptor D1A; Drd; Drd-; Drd-1; Drd1a; G protein-coupled receptor DRD1; Gpcr; Gpcr15.

Gene ID

13488

Accession Number

NP_001278730.1

Reactivity

Mouse, Mus musculus

Target

D (1A) dopamine receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DRD1 ELISA Kit (Mouse) (OKEH03336) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Drd1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Drd1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Drd1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

0.09 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Mouse D (1A) dopamine receptor

Protein Length

446

NCBI Gene Symbol

Drd1

Protein Name

Q61616

Gene Name URL

Drd1

Nucleotide Accession Number

NM_001291801.1

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