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CMKLR1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Chemerin chemokine-like receptor 1

Gene Aliases

Chemerin receptor; CHEMERINR; chemokine receptor-like 1; chemokine-like receptor 1; ChemR23; DEZ; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor, Dez; resolvin E1 receptor; RVER1.

Gene ID

1240

Accession Number

NP_001135815.1

Reactivity

Homo sapiens, Human

Target

Chemokine-like receptor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CMKLR1 ELISA Kit (Human) (OKEH03335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CMKLR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CMKLR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CMKLR1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

39.4 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Human Chemokine-like receptor 1

Protein Length

373

NCBI Gene Symbol

CMKLR1

Protein Name

Q99788

Gene Name URL

CMKLR1

Nucleotide Accession Number

NM_001142343.1

Frequently Asked Questions

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