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CMPK1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Cytidine/uridine monophosphate kinase 1

Gene Aliases

CK; CMK; CMPK; cytidine monophosphate (UMP-CMP) kinase 1, cytosolic; cytidylate kinase; dCMP kinase; deoxycytidylate kinase; nucleoside-diphosphate kinase; UMK; UMP/CMP kinase; UMP-CMP kinase; UMP-CMPK; UMPK; uridine monophosphate kinase; uridine monophosphate/cytidine monophosphate kinase.

Gene ID

51727

Accession Number

NP_001129612.1

Reactivity

Homo sapiens, Human

Target

UMP-CMP kinase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CMPK1 ELISA Kit (Human) (OKEH03334) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CMPK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CMPK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CMPK1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

Sensitivity

0.186 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

22 kDa

Specificity

Natural and recombinant Human UMP-CMP kinase

Protein Length

196

NCBI Gene Symbol

CMPK1

Protein Name

P30085

Gene Name URL

CMPK1

Nucleotide Accession Number

NM_001136140.1

Frequently Asked Questions

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