REC8 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
REC8 meiotic recombination protein
Gene Aliases
Cohesin Rec8p; cohesion rec8p; HR21spB; human homolog of rad21, S. pombe; kleisin-alpha; meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family; meiotic recombination protein REC8 homolog; meiotic recombination protein REC8-like 1; REC8 homolog; REC8L1; REC8-like 1; Rec8p; recombination and sister chromatid cohesion protein homolog.
Gene ID
9985
Accession Number
NP_001041670.1
Reactivity
Homo sapiens, Human
Target
Meiotic recombination protein REC8 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology REC8 ELISA Kit (Human) (OKEH03329) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human REC8 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated REC8 and incubated. The REC8 found in the sample or standards competes with the biotinylated REC8 for limited binding sites on the immobilized anti-Human REC8 antibody. Excess unbound biotinylated REC8 and sample or standard REC8 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated REC8 captured in the well and inversely proportional to the amount of REC8 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
63 kDa
Specificity
Natural and recombinant Human Meiotic recombination protein REC8 homolog
Protein Length
547
NCBI Gene Symbol
REC8
Protein Name
O95072
Gene Name URL
REC8
Nucleotide Accession Number
NM_001048205.1
Frequently Asked Questions
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