What is the catalog number for FTO ELISA Kit (Human)?

The catalog number for FTO ELISA Kit (Human) is OKEH03328-96W.

What sizes are available for FTO ELISA Kit (Human)?

FTO ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is FTO ELISA Kit (Human) in stock?

FTO ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

FTO ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

FTO alpha-ketoglutarate dependent dioxygenase

Gene Aliases

AlkB homolog 9; ALKBH9; alpha-ketoglutarate-dependent dioxygenase FTO; BMIQ14; fat mass and obesity associated; fat mass and obesity-associated protein; GDFD; m6A (m) -demethylase FTO; mRNA (2'-O-methyladenosine-N (6) -) -demethylase FTO; mRNA N (6) -methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N (6) -) -demethylase FTO; U6 small nuclear RNA N (6) -methyladenosine-demethylase FTO.

Gene ID

79068

Accession Number

NP_001073901.1

Reactivity

Homo sapiens, Human

Target

Alpha-ketoglutarate-dependent dioxygenase FTO

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FTO ELISA Kit (Human) (OKEH03328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FTO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for FTO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FTO captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol