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AR ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Androgen receptor

Gene Aliases

Andr; androgen receptor; androgen receptor (Testicular feminization), same as Tfm; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4; Tfm.

Gene ID

24208

Accession Number

NP_036634.1

Reactivity

Rat, Rattus norvegicus

Target

Androgen receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AR ELISA Kit (Rat) (OKEH03324) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ar has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ar is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ar captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into serum: 88%

Sensitivity

39.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

98 kDa

Notes

Formerly GWB-V05D6G

Specificity

Natural and recombinant Rat Androgen receptor

Protein Length

902

NCBI Gene Symbol

Ar

Protein Name

P15207

Gene Name URL

Ar

Nucleotide Accession Number

NM_012502.1

Frequently Asked Questions

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