FABP3 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Fatty acid binding protein 3, muscle and heart
Gene Aliases
Fab; Fabp; Fabph; Fabph1; Fabph-1; Fabph4; Fabph-4; fatty acid binding protein heart 1; fatty acid binding protein heart 4; fatty acid-binding protein 3; fatty acid-binding protein, heart; heart-type fatty acid-binding protein; H-FA; H-FABP; mammary-derived growth inhibitor; Mdg; Mdgi.
Gene ID
14077
Accession Number
NP_034304.1
Reactivity
Mouse, Mus musculus
Target
Fatty acid-binding protein, heart
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FABP3 ELISA Kit (Mouse) (OKEH03322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fabp3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Fabp3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fabp3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
15 kDa
Specificity
Natural and recombinant Mouse Fatty acid-binding protein, heart
Protein Length
133
NCBI Gene Symbol
Fabp3
Protein Name
P11404
Gene Name URL
Fabp3
Nucleotide Accession Number
NM_010174.1
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