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GOT1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Glutamic-oxaloacetic transaminase 1

Gene Aliases

AAT1; ASAT; aspartate aminotransferase 1; aspartate aminotransferase, cytoplasmic; Aspat; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Gaspat; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble; transaminase A.

Gene ID

24401

Accession Number

NP_036703.2

Reactivity

Rat, Rattus norvegicus

Target

Aspartate aminotransferase, cytoplasmic

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GOT1 ELISA Kit (Rat) (OKEH03319) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Got1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Got1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Got1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

3.12-200U/L

Sensitivity

1.63U/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

46 kDa

Specificity

Natural and recombinant Rat Aspartate aminotransferase, cytoplasmic

Protein Length

413

NCBI Gene Symbol

Got1

Protein Name

P13221

Gene Name URL

Got1

Nucleotide Accession Number

NM_012571.2

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