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GPER1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

G protein-coupled estrogen receptor 1

Gene Aliases

6330420K13Rik; Ce; Ceprl; chemoattractant receptor-like 2; CMKR; CMKRL2; constitutively expressed peptide-like receptor like; FEG-1; G protein-coupled estrogen receptor 1; Gp; GPCR; GPCR-Br; Gper; Gpr; Gpr30; G-protein coupled estrogen receptor 1; G-protein coupled receptor 30; membrane estrogen receptor; mER.

Gene ID

76854

Accession Number

NP_084047.2

Reactivity

Mouse, Mus musculus

Target

G-protein coupled estrogen receptor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GPER1 ELISA Kit (Mouse) (OKEH03310) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gper1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gper1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gper1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Mouse G-protein coupled estrogen receptor 1

Protein Length

375

NCBI Gene Symbol

Gper1

Protein Name

Q8BMP4

Gene Name URL

Gper1

Nucleotide Accession Number

NM_029771.3

Frequently Asked Questions

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