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IRF2BP2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Interferon regulatory factor 2 binding protein 2

Gene Aliases

CVID14; interferon regulatory factor 2-binding protein 2; IRF-2-binding protein 2.

Gene ID

359948

Accession Number

NP_001070865.1

Reactivity

Homo sapiens, Human

Target

Interferon regulatory factor 2-binding protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology IRF2BP2 ELISA Kit (Human) (OKEH03309) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human IRF2BP2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IRF2BP2 and incubated. The IRF2BP2 found in the sample or standards competes with the biotinylated IRF2BP2 for limited binding sites on the immobilized anti-Human IRF2BP2 antibody. Excess unbound biotinylated IRF2BP2 and sample or standard IRF2BP2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IRF2BP2 captured in the well and inversely proportional to the amount of IRF2BP2 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92.9%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

61 kDa

Specificity

Natural and recombinant Human Interferon regulatory factor 2-binding protein 2

Protein Length

587

NCBI Gene Symbol

IRF2BP2

Protein Name

Q7Z5L9

Gene Name URL

IRF2BP2

Nucleotide Accession Number

NM_001077397.1

Frequently Asked Questions

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