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FAM40A ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Striatin interacting protein 1

Gene Aliases

FAM40A; family with sequence similarity 40, member A; FAR11 factor arrest 11 homolog A; FAR11A; homolog of yeast FAR11 protein 1; protein FAM40A; striatin-interacting protein 1.

Gene ID

85369

Accession Number

NP_001257697.1

Reactivity

Homo sapiens, Human

Target

Striatin-interacting protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology FAM40A ELISA Kit (Human) (OKEH03301) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human FAM40A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FAM40A and incubated. The FAM40A found in the sample or standards competes with the biotinylated FAM40A for limited binding sites on the immobilized anti-Human FAM40A antibody. Excess unbound biotinylated FAM40A and sample or standard FAM40A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FAM40A captured in the well and inversely proportional to the amount of FAM40A which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%

Sensitivity

0.396 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

96 kDa

Specificity

Natural and recombinant Human Protein FAM40A

Protein Length

837

NCBI Gene Symbol

STRIP1

Protein Name

Q5VSL9

Gene Name URL

STRIP1

Nucleotide Accession Number

NM_001270768.1

Frequently Asked Questions

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