FAM40A ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Striatin interacting protein 1
Gene Aliases
FAM40A; family with sequence similarity 40, member A; FAR11 factor arrest 11 homolog A; FAR11A; homolog of yeast FAR11 protein 1; protein FAM40A; striatin-interacting protein 1.
Gene ID
85369
Accession Number
NP_001257697.1
Reactivity
Homo sapiens, Human
Target
Striatin-interacting protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology FAM40A ELISA Kit (Human) (OKEH03301) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human FAM40A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FAM40A and incubated. The FAM40A found in the sample or standards competes with the biotinylated FAM40A for limited binding sites on the immobilized anti-Human FAM40A antibody. Excess unbound biotinylated FAM40A and sample or standard FAM40A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FAM40A captured in the well and inversely proportional to the amount of FAM40A which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.396 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
96 kDa
Specificity
Natural and recombinant Human Protein FAM40A
Protein Length
837
NCBI Gene Symbol
STRIP1
Protein Name
Q5VSL9
Gene Name URL
STRIP1
Nucleotide Accession Number
NM_001270768.1
Frequently Asked Questions
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