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C1QTNF3 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

C1q and tumor necrosis factor related protein 3

Gene Aliases

2310005P21Rik; Adim; adiponectin m; cartducin; cartonectin; Co; collagenous repeat-containing sequence 26 kDa protein; complement C1q tumor necrosis factor-related protein 3; Corcs; Cors; CORS26; CORS-26; CTR; CTRP3; secretory protein CORS26.

Gene ID

81799

Accession Number

NP_112150.1

Reactivity

Mouse, Mus musculus

Target

Complement C1q tumor necrosis factor-related protein 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology C1QTNF3 ELISA Kit (Mouse) (OKEH03291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qtnf3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for C1qtnf3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1qtnf3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

15.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

27 kDa

Specificity

Natural and recombinant Mouse Complement C1q tumor necrosis factor-related protein 3

Protein Length

246

NCBI Gene Symbol

C1qtnf3

Protein Name

Q9ES30

Gene Name URL

C1qtnf3

Nucleotide Accession Number

NM_030888.4

Frequently Asked Questions

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