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CALB1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Calbindin 1

Gene Aliases

CaBP28K; calbindin; calbindin 1, 28 kD; calbindin D28; cerebellar Ca-binding protein, spot 35 protein; D-28K; spot 35 protein; vitamin D-dependent calcium-binding protein, avian-type.

Gene ID

83839

Accession Number

NP_114190.1

Reactivity

Rat, Rattus norvegicus

Target

Calbindin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CALB1 ELISA Kit (Rat) (OKEH03287) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calb1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Calb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Calb1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Sensitivity

7.84 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

30 kDa

Specificity

Natural and recombinant Rat Calbindin

Protein Length

261

NCBI Gene Symbol

Calb1

Protein Name

P07171

Gene Name URL

Calb1

Nucleotide Accession Number

NM_031984.2

Frequently Asked Questions

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