What is the catalog number for ALPL ELISA Kit (Rat)?

The catalog number for ALPL ELISA Kit (Rat) is OKEH03286-96W.

What sizes are available for ALPL ELISA Kit (Rat)?

ALPL ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is ALPL ELISA Kit (Rat) in stock?

ALPL ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

ALPL ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Alkaline phosphatase, biomineralization associated

Gene Aliases

Akp2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; PHOA; tissue-nonspecific alkaline phosphatase; Tissue-nonspecific ALP alkaline phosphatase; TNSALP.

Gene ID

25586

Accession Number

NP_037191.1

Reactivity

Rat, Rattus norvegicus

Target

Alkaline phosphatase, tissue-nonspecific isozyme

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biologyâ€™s ALPL ELISA Kit (Human) (OKEH03286) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALPL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALPL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALPL captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol