ABCC1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
ATP binding cassette subfamily C member 1
Gene Aliases
Abcc1a; ATP-binding cassette sub-family C (CFTR/MRP) member 1a; ATP-binding cassette sub-family C member 1; ATP-binding cassette, subfamily C (CFTR/MRP), member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette, sub-family C, member 1-like; Avcc1a; glutathione-S-conjugate-translocating ATPase ABCC1; leukotriene C (4) transporter; LTC4 transporter; Mrp; Mrp1; multidrug resistance protein 1; multidrug resistance-associated protein 1; multidrug resistance-associated protein 1-like; multiple drug resistance-associated protein.
Gene ID
24565
Accession Number
NP_071617.2
Reactivity
Rat, Rattus norvegicus
Target
Multidrug resistance-associated protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ABCC1 ELISA Kit (Rat) (OKEH03279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abcc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Abcc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abcc1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
<= 5.3%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
171 kDa
Specificity
Natural and recombinant Rat Multidrug resistance-associated protein 1
Protein Length
1532
NCBI Gene Symbol
Abcc1
Protein Name
Q8CG09
Gene Name URL
Abcc1
Nucleotide Accession Number
NM_022281.2
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