ABCB11 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
ATP binding cassette subfamily B member 11
Gene Aliases
ABC member 16, MDR/TAP subfamily; ABC16; ATP-binding cassette sub-family B member 11; ATP-binding cassette, sub-family B (MDR/TAP), member 11; bile salt export pump; BRIC2; BSEP; PFIC2; PFIC-2; PGY4; progressive familial intrahepatic cholestasis 2; sister p-glycoprotein; SPGP.
Gene ID
8647
Accession Number
NP_003733.2
Reactivity
Homo sapiens, Human
Target
Bile salt export pump
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ABCB11 ELISA Kit (Human) (OKEH03272) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCB11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ABCB11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCB11 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
40 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Human Bile salt export pump
Protein Length
104
NCBI Gene Symbol
ABCB11
Protein Name
O95342
Gene Name URL
ABCB11
Nucleotide Accession Number
NM_003742.2
Frequently Asked Questions
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