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ENPP1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Ectonucleotide pyrophosphatase/phosphodiesterase 1

Gene Aliases

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Npps; Pc1; phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein PC-1.

Gene ID

85496

Accession Number

NP_445987.1

Reactivity

Rat, Rattus norvegicus

Target

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ENPP1 ELISA Kit (Rat) (OKEH03269) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Enpp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Enpp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Enpp1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Sensitivity

15.67 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

103 kDa

Specificity

Natural and recombinant Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Protein Length

906

NCBI Gene Symbol

Enpp1

Protein Name

Q924C3

Gene Name URL

Enpp1

Nucleotide Accession Number

NM_053535.1

Frequently Asked Questions

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