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PROK2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Prokineticin 2

Gene Aliases

Bombina variegata 8 kD protein; Bombina variegata 8kDa protein; Bv; Bv 8 homolog (Bombina variegata) ; Bv8; PK; PK2; Prok1; prokineticin 1; prokineticin-2; protein Bv8 homolog.

Gene ID

50501

Accession Number

NP_001032628.1

Reactivity

Mouse, Mus musculus

Target

Prokineticin-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology PROK2 ELISA Kit (Mouse) (OKEH03268) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prok2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prok2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prok2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

0.164 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

14 kDa

Specificity

Natural and recombinant Mouse Prokineticin-2

Protein Length

128

NCBI Gene Symbol

Prok2

Protein Name

Q9QXU7

Gene Name URL

Prok2

Nucleotide Accession Number

NM_001037539.2

Frequently Asked Questions

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