PCK1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Phosphoenolpyruvate carboxykinase 1, cytosolic
Gene Aliases
AI265463; Pck-; Pck-1; PEP; PEPCK; PEPCK-C; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; serine-protein kinase PCK1.
Gene ID
18534
Accession Number
NP_035174.1
Reactivity
Mouse, Mus musculus
Target
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PCK1 ELISA Kit (Mouse) (OKEH03250) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pck1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pck1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pck1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
69 kDa
Specificity
Natural and recombinant Mouse Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Protein Length
622
NCBI Gene Symbol
Pck1
Protein Name
Q9Z2V4
Gene Name URL
Pck1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_011044.2
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