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ACP5 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Acid phosphatase 5, tartrate resistant

Gene Aliases

Acid phosphatase type V; T; tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase type 5; TR; TRACP; TRAP; TR-AP; trATPase; type 5 acid phosphatase.

Gene ID

11433

Accession Number

NP_001095874.1

Reactivity

Mouse, Mus musculus

Target

Tartrate-resistant acid phosphatase type 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ACP5 ELISA Kit (Mouse) (OKEH03244) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Acp5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Acp5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Acp5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20U/L

Sensitivity

0.156U/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

37 kDa

Specificity

Natural and recombinant Mouse Tartrate-resistant acid phosphatase type 5

Protein Length

327

NCBI Gene Symbol

Acp5

Protein Name

Q05117

Gene Name URL

Acp5

Nucleotide Accession Number

NM_001102404.1

Frequently Asked Questions

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