LIMK2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
LIM domain kinase 2
Gene Aliases
LIM domain kinase 2.
Gene ID
3985
Accession Number
NP_001026971.1
Reactivity
Homo sapiens, Human
Target
LIM domain kinase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology LIMK2 ELISA Kit (Human) (OKEH03219) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LIMK2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated LIMK2 and incubated. The LIMK2 found in the sample or standards competes with the biotinylated LIMK2 for limited binding sites on the immobilized anti-Human LIMK2 antibody. Excess unbound biotinylated LIMK2 and sample or standard LIMK2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated LIMK2 captured in the well and inversely proportional to the amount of LIMK2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93.9%
Sensitivity
0.43 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
72 kDa
Specificity
Natural and recombinant Human LIM domain kinase 2
Protein Length
638
NCBI Gene Symbol
LIMK2
Protein Name
P53671
Gene Name URL
LIMK2
Nucleotide Accession Number
NM_001031801.1
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