What is the catalog number for LIMK2 ELISA Kit (Human)?

The catalog number for LIMK2 ELISA Kit (Human) is OKEH03219-96W.

What sizes are available for LIMK2 ELISA Kit (Human)?

LIMK2 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is LIMK2 ELISA Kit (Human) in stock?

LIMK2 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

LIMK2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

LIM domain kinase 2

Gene Aliases

LIM domain kinase 2.

Gene ID

3985

Accession Number

NP_001026971.1

Reactivity

Homo sapiens, Human

Target

LIM domain kinase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology LIMK2 ELISA Kit (Human) (OKEH03219) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LIMK2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated LIMK2 and incubated. The LIMK2 found in the sample or standards competes with the biotinylated LIMK2 for limited binding sites on the immobilized anti-Human LIMK2 antibody. Excess unbound biotinylated LIMK2 and sample or standard LIMK2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated LIMK2 captured in the well and inversely proportional to the amount of LIMK2 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol