What is the catalog number for HCRT ELISA Kit (Rat)?

The catalog number for HCRT ELISA Kit (Rat) is OKEH03216-96W.

What sizes are available for HCRT ELISA Kit (Rat)?

HCRT ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is HCRT ELISA Kit (Rat) in stock?

HCRT ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

HCRT ELISA Kit (Rat)

Selected Variant

OKEH03216-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Hypocretin neuropeptide precursor

Gene Aliases

Hypocretin; Hypocretin (orexin) neuropeptide; orexin; orexin-A.

Gene ID

25723

Accession Number

NP_037311.1

Reactivity

Rat, Rattus norvegicus

Target

Orexin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HCRT ELISA Kit (Rat) (OKEH03216) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hcrt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hcrt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hcrt captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

7.8-500pg/mL

Sensitivity

3.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

14 kDa

Specificity

Natural and recombinant Rat Orexin

Protein Length

130

NCBI Gene Symbol

Hcrt

Protein Name

O55232

Gene Name URL

Hcrt

Nucleotide Accession Number

NM_013179.2

Price

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