What is the catalog number for PFKM ELISA Kit (Human)?

The catalog number for PFKM ELISA Kit (Human) is OKEH03204-96W.

What sizes are available for PFKM ELISA Kit (Human)?

PFKM ELISA Kit (Human) is available in the following size(s): 96 Well.

Is PFKM ELISA Kit (Human) in stock?

PFKM ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

PFKM ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Phosphofructokinase, muscle

Gene Aliases

6-phosphofructo-1-kinase;6-phosphofructokinase type A;6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; GSD7; PFK1; PFK-1; PFKA; PFK-A; PFKX; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase, polypeptide X; phosphofructokinase-M; phosphohexokinase; PPP1R122; protein phosphatase 1, regulatory subunit 122.

Gene ID

5213

Accession Number

NP_000280.1

Reactivity

Homo sapiens, Human

Target

ATP-dependent 6-phosphofructokinase, muscle type

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PFKM ELISA Kit (Human) (OKEH03204) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PFKM has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for PFKM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PFKM captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol