What is the catalog number for F2 ELISA Kit (Rat)?

The catalog number for F2 ELISA Kit (Rat) is OKEH03200-96W.

What sizes are available for F2 ELISA Kit (Rat)?

F2 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is F2 ELISA Kit (Rat) in stock?

F2 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

F2 ELISA Kit (Rat)

Selected Variant

OKEH03200-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Coagulation factor II

Gene Aliases

Coagulation factor II.

Gene ID

29251

Accession Number

NP_075213.2

Reactivity

Rat, Rattus norvegicus

Target

Prothrombin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology F2 ELISA Kit (Rat) (OKEH03200) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for F2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

1.56-100ng/mL

Sensitivity

0.81 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

70 kDa

Specificity

Natural and recombinant Rat Prothrombin

Protein Length

617

NCBI Gene Symbol

Protein Name

P18292

Gene Name URL

Nucleotide Accession Number

NM_022924.2Â

Price

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