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PRDX5 ELISA Kit (Rat)

Selected Variant

OKEH03197-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Peroxiredoxin 5

Gene Aliases

Antioxidant enzyme B166; Aoeb166; peroxiredoxin V; peroxiredoxin-5, mitochondrial; peroxisomal antioxidant enzyme; PLP; prx-V; thioredoxin peroxidase PMP20; thioredoxin reductase; thioredoxin-dependent peroxiredoxin 5.

Gene ID

113898

Accession Number

NP_446062.1

Reactivity

Rat, Rattus norvegicus

Target

Peroxiredoxin-5, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PRDX5 ELISA Kit (Rat) (OKEH03197) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Prdx5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prdx5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.78-50ng/mL

Sensitivity

0.453 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

22 kDa

Specificity

Natural and recombinant Rat Peroxiredoxin-5, mitochondrial

Protein Length

213

NCBI Gene Symbol

Prdx5

Protein Name

Q9R063

Gene Name URL

Prdx5

Nucleotide Accession Number

NM_053610.1

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