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RCAN1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Regulator of calcineurin 1

Gene Aliases

ADAPT78; calcipressin-1; calcium and oxidant-inducible mRNA; CSP1; Down syndrome candidate region 1; Down syndrome critical region gene 1; Down syndrome critical region protein 1; DSC1; DSCR1; MCIP1; modulatory calcineurin-interacting protein 1; myocyte-enriched calcineurin-interacting protein 1; near DSCR proline-rich protein; RCN1; Regulator of calcineurin 1.

Gene ID

1827

Accession Number

NP_001272318.1

Reactivity

Homo sapiens, Human

Target

Calcipressin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RCAN1 ELISA Kit (Human) (OKEH03190) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RCAN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for RCAN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RCAN1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

28 kDa

Specificity

Natural and recombinant Human Calcipressin-1

Protein Length

252

NCBI Gene Symbol

RCAN1

Protein Name

P53805

Gene Name URL

RCAN1

Nucleotide Accession Number

NM_001285389.2

Frequently Asked Questions

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