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LPAR4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Lysophosphatidic acid receptor 4

Gene Aliases

5730485F04Rik; G protein-coupled receptor 23; Gpr; Gpr23; G-protein coupled receptor 23; L; LPA receptor 4; LPA4; LPA-4; lysophosphatidic acid receptor 4; p2y; P2Y purinoceptor 9; p2y9; purinergic receptor 9.

Gene ID

78134

Accession Number

NP_780480.2

Reactivity

Mouse, Mus musculus

Target

Lysophosphatidic acid receptor 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LPAR4 ELISA Kit (Mouse) (OKEH03171) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lpar4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Lpar4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lpar4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Mouse Lysophosphatidic acid receptor 4

Protein Length

370

NCBI Gene Symbol

Lpar4

Protein Name

Q8BLG2

Gene Name URL

Lpar4

Nucleotide Accession Number

NM_175271.4

Frequently Asked Questions

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