ITGA2B ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Integrin alpha 2b
Gene Aliases
AI172977; al; alpha IIb; alphaIIb; CD41; CD41B; Gp; GP IIb; GPalpha IIb; GpIIb; integrin alpha-IIb; platelet glycoprotein IIb of IIb/II Ia complex; platelet membrane glycoprotein IIb.
Gene ID
16399
Accession Number
NP_034705.2
Reactivity
Mouse, Mus musculus
Target
Integrin alpha-IIb
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ITGA2B ELISA Kit (Mouse) (OKEH03165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Itga2b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Itga2b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Itga2b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
113 kDa
Specificity
Natural and recombinant Mouse Integrin alpha-IIb
Protein Length
1033
NCBI Gene Symbol
Itga2b
Protein Name
Q9QUM0
Gene Name URL
Itga2b
Nucleotide Accession Number
NM_010575.2
Frequently Asked Questions
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