GZMB ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Granzyme B, Granzyme B-like 2
Gene Aliases
Fragmentin; GLP I; GLP III; GLP-1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) ; granzyme-like protein 1; Granzyme-like protein 1-like; granzyme-like protein I; natural killer cell protease 1; RNKP-1.
Gene ID
100361468, 171528
Accession Number
NP_612526.2
Reactivity
Rat, Rattus norvegicus
Target
Granzyme B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GZMB ELISA Kit (Rat) (OKEH03163) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gzmb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gzmb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gzmb captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Sensitivity
7.86 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
27 kDa
Specificity
Natural and recombinant Rat Granzyme B
Protein Length
248
NCBI Gene Symbol
Gzmb, Gzmbl2
Protein Name
P18291
Gene Name URL
Gzmb, Gzmbl2
Nucleotide Accession Number
NM_138517.3
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