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MLPH ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Melanophilin

Gene Aliases

Exophilin-3; melanophilin; SLAC2-A; slp homolog lacking C2 domains a; synaptotagmin-like protein 2a.

Gene ID

79083

Accession Number

NP_001035932.1

Reactivity

Homo sapiens, Human

Target

Melanophilin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MLPH ELISA Kit (Human) (OKEH03150) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MLPH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for MLPH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MLPH captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

66 kDa

Specificity

Natural and recombinant Human Melanophilin

Protein Length

600

NCBI Gene Symbol

MLPH

Protein Name

Q9BV36

Gene Name URL

MLPH

Nucleotide Accession Number

NM_001042467.2

Frequently Asked Questions

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