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ENO2 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Enolase 2, gamma-enolase-like

Gene Aliases

2-phospho-D-glycerate hydro-lyase; Enolase 2; enolase 2, gamma, neuronal; gamma-enolase; gamma-enolase-like; neural enolase; neuron-specific enolase; NSE; RNEN3.

Gene ID

100911625, 24334

Accession Number

NP_647541.1

Reactivity

Rat, Rattus norvegicus

Target

Gamma-enolase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ENO2 ELISA Kit (Rat) (OKEH03139) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eno2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Eno2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eno2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.084 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

47 kDa

Specificity

Natural and recombinant Rat Gamma-enolase

Protein Length

434

NCBI Gene Symbol

Eno2, LOC100911625

Protein Name

P07323

Gene Name URL

Eno2, LOC100911625

Nucleotide Accession Number

NM_139325.3

Frequently Asked Questions

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