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BTNL2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Butyrophilin like 2

Gene Aliases

BTL-II; BTN7; butyrophilin-like 2 (MHC class II associated) ; butyrophilin-like protein 2; HSBLMHC1; SS2; truncated BTNL2.

Gene ID

56244

Accession Number

NP_001291490.1

Reactivity

Homo sapiens, Human

Target

Butyrophilin-like protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology BTNL2 ELISA Kit (Human) (OKEH03136) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human BTNL2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated BTNL2 and incubated. The BTNL2 found in the sample or standards competes with the biotinylated BTNL2 for limited binding sites on the immobilized anti-human BTNL2 antibody. Excess unbound biotinylated BTNL2 and sample or standard BTNL2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated BTNL2 captured in the well and inversely proportional to the amount of BTNL2 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.164 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Human Butyrophilin-like protein 2

Protein Length

455

NCBI Gene Symbol

BTNL2

Protein Name

Q9UIR0

Gene Name URL

BTNL2

Nucleotide Accession Number

NM_001304561.1

Frequently Asked Questions

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