BTNL2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Butyrophilin like 2
Gene Aliases
BTL-II; BTN7; butyrophilin-like 2 (MHC class II associated) ; butyrophilin-like protein 2; HSBLMHC1; SS2; truncated BTNL2.
Gene ID
56244
Accession Number
NP_001291490.1
Reactivity
Homo sapiens, Human
Target
Butyrophilin-like protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology BTNL2 ELISA Kit (Human) (OKEH03136) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human BTNL2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated BTNL2 and incubated. The BTNL2 found in the sample or standards competes with the biotinylated BTNL2 for limited binding sites on the immobilized anti-human BTNL2 antibody. Excess unbound biotinylated BTNL2 and sample or standard BTNL2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated BTNL2 captured in the well and inversely proportional to the amount of BTNL2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.164 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Human Butyrophilin-like protein 2
Protein Length
455
NCBI Gene Symbol
BTNL2
Protein Name
Q9UIR0
Gene Name URL
BTNL2
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001304561.1
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