HBA1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Hemoglobin alpha, adult chain 1, hemoglobin alpha, adult chain 2
Gene Aliases
2-alpha globin;2-alpha-1 globin; alpha-1/2-globin; Glnc2; Glnc3; globin c2; globin c3; Hba1; Hba2; Hba-a1; Hba-a2; HBAM; hemoglobin alpha 1 chain; hemoglobin alpha 2 chain; hemoglobin alpha, adult chain 2; hemoglobin alpha-1/2 chain; hemoglobin subunit alpha-1/2; hemoglobin, alpha 1; hemoglobin, alpha 2.
Gene ID
25632, 360504
Accession Number
NP_001007723.1
Reactivity
Rat, Rattus norvegicus
Target
Hemoglobin subunit alpha-1/2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HBA1 ELISA Kit (Rat) (OKEH03135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hba1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hba1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hba1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
1.59 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
15 kDa
Specificity
Natural and recombinant Rat Hemoglobin subunit alpha-1/2
Protein Length
142
NCBI Gene Symbol
Hba-a1, Hba-a2
Protein Name
P01946
Gene Name URL
Hba-a1, Hba-a2
Nucleotide Accession Number
NM_001007722.1
Frequently Asked Questions
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