SLC25A5 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 5
Gene Aliases
Adenine nucleotide translocase; Adenine nucleotide translocator 2; adenine nucleotide translocator 2, fibroblast; ADP, ATP carrier protein 2; ADP, ATP carrier protein 2; ADP/ATP translocase 2; An; Ant2; Solute carrier family 25 member 5.
Gene ID
11740
Accession Number
NP_031477.1
Reactivity
Mouse, Mus musculus
Target
ADP/ATP translocase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SLC25A5 ELISA Kit (Mouse) (OKEH03131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc25a5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Slc25a5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc25a5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
33 kDa
Specificity
Natural and recombinant Mouse ADP/ATP translocase 2
Protein Length
298
NCBI Gene Symbol
Slc25a5
Protein Name
P51881
Gene Name URL
Slc25a5
CAS Number
7732-18-5
Nucleotide Accession Number
NM_007451.4
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