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REG3B ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Regenerating islet-derived 3 beta

Gene Aliases

HIP; P; pancreatitis-associated protein 1; Pap; PAP1; REG; reg III-beta; REG-3-beta; regenerating islet-derived protein 3-beta; regenerating islet-derived protein III-beta; REG-III; RegIII (beta) .

Gene ID

18489

Accession Number

NP_035166.1

Reactivity

Mouse, Mus musculus

Target

Regenerating islet-derived protein 3-beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology REG3B ELISA Kit (Mouse) (OKEH03109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Reg3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Reg3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Reg3b captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117%

Sensitivity

0.424 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Specificity

Natural and recombinant Mouse Regenerating islet-derived protein 3-beta

Protein Length

175

NCBI Gene Symbol

Reg3b

Protein Name

P35230

Gene Name URL

Reg3b

Nucleotide Accession Number

NM_011036.1

Frequently Asked Questions

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