KLK9 ELISA Kit (Rat)
Selected Variant
OKEH03108-96W
Size: 96 Well
Product Specifications
CAS Number
7732-18-5
Gene Name
Kallikrein 1-related peptidase C9
Gene Aliases
Kallikrein a2; kallikrein, submaxillary gland S3; Klk9; Klks3; KLK-S3; Klna2; rGK-9; rK9; S3 kallikrein; SEV; submandibular enzymatic vasoconstrictor; submandibular glandular kallikrein-9; submaxillary gland S3 kallikrein; tissue kallikrein.
Gene ID
292868
Accession Number
NP_786935.1
Reactivity
Rat, Rattus norvegicus
Target
Submandibular glandular kallikrein-9
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology KLK9 ELISA Kit (Rat) (OKEH03108) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Klk9 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Klk9 and incubated. The Klk9 found in the sample or standards competes with the biotinylated Klk9 for limited binding sites on the immobilized anti-rat Klk9 antibody. Excess unbound biotinylated Klk9 and sample or standard Klk9 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Klk9 captured in the well and inversely proportional to the amount of Klk9 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Rat Submandibular glandular kallikrein-9
Protein Length
259
NCBI Gene Symbol
Klk1c9
Protein Name
P07647
Gene Name URL
Klk1c9
Nucleotide Accession Number
NM_175759.2
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