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MMP12 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Matrix metallopeptidase 12

Gene Aliases

Macrophage metalloelastase; matrix metalloproteinase-12; Mme; MMP-12.

Gene ID

117033

Accession Number

NP_446415.2

Reactivity

Rat, Rattus norvegicus

Target

Macrophage metalloelastase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MMP12 ELISA Kit (Rat) (OKEH03106) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Mmp12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp12 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

54 kDa

Specificity

Natural and recombinant Rat Macrophage metalloelastase

Protein Length

465

NCBI Gene Symbol

Mmp12

Protein Name

Q63341

Gene Name URL

Mmp12

Nucleotide Accession Number

NM_053963.2

Frequently Asked Questions

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