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HNRNPK ELISA Kit (Mouse)

Selected Variant

OKEH03096-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Heterogeneous nuclear ribonucleoprotein K

Gene Aliases

Heterogeneous nuclear ribonucleoprotein K; hn; Hnrpk; KBB; KBBP; NOVA.

Gene ID

15387

Accession Number

NP_001288269.1

Reactivity

Mouse, Mus musculus

Target

Heterogeneous nuclear ribonucleoprotein K

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HNRNPK ELISA Kit (Mouse) (OKEH03096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hnrnpk has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hnrnpk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hnrnpk captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

40 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

51 kDa

Specificity

Natural and recombinant Mouse Heterogeneous nuclear ribonucleoprotein K

Protein Length

463

NCBI Gene Symbol

Hnrnpk

Protein Name

P61979

Gene Name URL

Hnrnpk

Nucleotide Accession Number

NM_001301340.1

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